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A Model system to study antimicrobial strategies in endodontic biofilms

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A Model system to study antimicrobial strategies in endodontic biofilms

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Título A Model system to study antimicrobial strategies in endodontic biofilms
Autor Estrela, Carlos
Sydney, Gilson Blitzkow
Figueiredo, Jose Antonio Poli de
Estrela, Cyntia Rodrigues de Araújo
Abstract The purpose of this work was to develop a model system to study antimicrobial strategies in endodontic biofilms. Enterococcus faecalis suspension was colonized in 10 human root canals. Five milliliters of Brain Heart Infusion (BHI) were mixed with 5 mL of the bacterial inoculums (E. faecalis) and inoculated with sufficient volume to fill the root canal during 60 days. This procedure was repeated every 72 h, always using 24-h pure culture prepared and adjusted to No. 1 MacFarland turbidity standard. Biofilm formation was analyzed by scanning electron microscopy (SEM). E. faecalis consistently adhered to collagen structure, colonized dentin surface, progressed towards the dentinal tubules and formed a biofilm. The proposed biofilm model seems to be viable for studies on antimicrobial strategies, and allows for a satisfactory colonization time of selected bacterial species with virulence and adherence properties.
Contido em Journal of applied oral science. Bauru. Vol. 17, no. 2 (Mar./Apr. 2009), p. 87-91
Assunto Antimicrobianos
Biofilmes
Canais radiculares
Endodontia
Enterococcus faecalis
[en] Antimicrobial strategies
[en] Biofilm
[en] Endodontic biofilm
[en] Enterococcus faecalis
[en] Intracanal medicaments
Origem Nacional
Tipo Artigo de periódico
URI http://hdl.handle.net/10183/61173
Arquivos Descrição Formato
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