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Analysis of the ntpdase and ecto-5´-nucleotidase profiles in serum-limited trichomonas vaginalis

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Analysis of the ntpdase and ecto-5´-nucleotidase profiles in serum-limited trichomonas vaginalis

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Título Analysis of the ntpdase and ecto-5´-nucleotidase profiles in serum-limited trichomonas vaginalis
Autor Frasson, Amanda Piccoli
Charão, Mariele Feiffer
Rosemberg, Denis Broock
Souza, Ana Paula de
Garcia, Solange Cristina
Bonorino, Cristina Beatriz Cazabuena
Bogo, Mauricio Reis
De Carli, Geraldo Attilio
Tasca, Tiana
Abstract Trichomonas vaginalis is a parasite of the human urogenital tract that causes trichomonosis, the most prevalent non-viral sexually transmitted disease. Ectonucleoside triphosphate diphosphohydrolase (NTPDase) family mem- bers, which hydrolyse extracellular ATP and ADP and ecto-5′-nucleotidase, which hydrolyses AMP, have been char- acterised in T. vaginalis. For trichomonad culture, the growth medium is supplemented with 10% serum, which is an important source of nutrients, such as adenosine. Here, we investigated the ATP metabolism of T. vaginalis tropho- zoites from long-term cultures and clinical isolates under limited bovine serum conditions (1% serum). The specific enzymatic activities were expressed as nmol inorganic phosphate (Pi) released/min/mg protein, the gene expression patterns were determined by reverse transcriptase-polymerase chain reaction, the extracellular adenine nucleotide hydrolysis was analysed by high performance liquid chromatography and the cell cycle analysis was assessed by f low cytometry. Serum limitation led to the profound activation of NTPDase and ecto-5’-nucleotidase activities. Furthermore, the levels of NTPDase A and B transcripts increased and extracellular ATP metabolism was activated, which led to enhanced ATP hydrolysis and the formation of ADP and AMP. Moreover, the cell cycle was arrested at the G0/G1 stage, which suggested adenosine uptake. Our data suggest that under conditions of serum limitation, NTPDase and ecto-5’-nucleotidase play a role in providing the adenosine required for T. vaginalis growth and that this process contributes to the establishment of parasitism.
Contido em Memórias do Instituto Oswaldo Cruz : an international journal of biological and biomedical research. Vol. 107, n. 2 (mar. 2012), p. 170-177
Assunto Adenosina
Ecto-5'-nucleotidase
Trichomonas vaginalis
[en] Adenosine
[en] Ecto-5’
[en] Ectonucleoside triphosphate diphosphohydrolase
[en] Nucleotidase
[en] Serum limitation
[en] Trichomonas vaginalis
Origem Nacional
Tipo Artigo de periódico
URI http://hdl.handle.net/10183/72280
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