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Detection of Cryptosporidium oocysts by auramine and Ziehl Neelsen staining methods

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Detection of Cryptosporidium oocysts by auramine and Ziehl Neelsen staining methods

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Título Detection of Cryptosporidium oocysts by auramine and Ziehl Neelsen staining methods
Autor Quadros, Rosiléia Marinho de
Marques, Sandra Marcia Tietz
Amendoeira, Camila
Souza, Larissa
Amendoeira, Paula
Comparin, Carla C.
Abstract Cryptosporidium spp is a common intestinal pathogen of animals and humans. It may have an important economic impact on farms and cause potentially zoonotic infections. Fecal specimens were collected from 331 domestic animals (81 beef cattle, 50 sheep, 100 pigs and 100 dogs) and checked for the presence of Cryptosporidium oocysts by way of Ziehl Neelsen and auramine staining methods. An overall positivity rate of 7.5% (25/331) was found, with rates of 10% (10/100) among the dogs and 18.5% (15/81) among the beef cattle. The feces of sheep and pigs tested negative. In beef cattle, 15 and 12 positive samples were detected by the auramine and Ziehl Neelsen staining techniques, respectively, with no statistically significant difference between the two methods. In dogs, the same number of positive samples was found by both techniques.
Contido em Parasitología latinoamericana. [Santiago, Chile]. Vol. 61, no. 3/4 (2006), p. 117-120
Assunto Animais domesticos
Cryptosporidium
Parasitologia
[en] Auramine
[en] Domestic animals
[en] Ziehl Neelsen method
Origem Nacional
Tipo Artigo de periódico
URI http://hdl.handle.net/10183/72314
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