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dc.contributor.authorBaierle, Maríliapt_BR
dc.contributor.authorBairros, André Valle dept_BR
dc.contributor.authorMoreira, Ana Paula L.pt_BR
dc.contributor.authorBulcão, Rachel Picadapt_BR
dc.contributor.authorRoehrs, Miguelpt_BR
dc.contributor.authorFreitas, Fernando dept_BR
dc.contributor.authorDurgante, Julianopt_BR
dc.contributor.authorBrucker, Natáliapt_BR
dc.contributor.authorCharão, Mariele Feifferpt_BR
dc.contributor.authorGarcia, Solange Cristinapt_BR
dc.date.accessioned2013-07-02T01:43:51Zpt_BR
dc.date.issued2012pt_BR
dc.identifier.issn0100-4042pt_BR
dc.identifier.urihttp://hdl.handle.net/10183/72945pt_BR
dc.description.abstractVitamin C, an exogenous antioxidant, is essential to human health. In this study, a method was validated to serum vitamin C quantification by HPLC-UV. Its stability with and without the use of tris [2-carboxy-ethyl] phosphine hydrochloride (TCEP), at-20 and -80 ºC, in serum and supernatant were also evaluated. Analysis showed r2 > 0.99, precision CV% < 15% and % bias < 15%, being linear, precise and accurate. The stability test revealed that using TCEP in serum storage at-20 and -80 ºC or in supernatant at -80 ºC the vitamin C levels remain stable for 30 and 12 days, respectively.en
dc.format.mimetypeapplication/pdf
dc.language.isoporpt_BR
dc.relation.ispartofQuímica nova. São Paulo: Sociedade Brasileira de Química, 1978-. Vol. 35, n. 2, (2012), p. 403-407pt_BR
dc.rightsOpen Accessen
dc.subjectHPLCen
dc.subjectVitamina Cpt_BR
dc.subjectCromatografia liquida de alta eficiencia (clae)pt_BR
dc.subjectVitamin Cen
dc.subjectEstabilidadept_BR
dc.subjectStabilityen
dc.titleQuantificação sérica de vitamina c por clae-uv e estudo de estabilidadept_BR
dc.title.alternativeSerum quantification of vitamin c by hplc-uv and stability study en
dc.typeArtigo de periódicopt_BR
dc.identifier.nrb000873523pt_BR
dc.type.originNacionalpt_BR


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