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Molecular techniques for the study and diagnosis of parasite infection

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Molecular techniques for the study and diagnosis of parasite infection

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Título Molecular techniques for the study and diagnosis of parasite infection
Autor Tavares, R.G.
Staggemeier, Rodrigo
Borges, A.L.P.
Rodrigues, M.T.
Castelan, L.A.
Vasconcelos, J.
Anschau, M.E.
Spalding, Silvia Maria
Abstract In parasitology, routine laboratory diagnosis involves conventional methods, such as optical microscopy, used for the morphological identification of parasites. Currently, molecular biology techniques are increasingly used to diagnose parasite structures in order to enhance the identification and characterization of parasites. The objective of the present study was to review the main current and new diagnostic techniques for confirmation of parasite infections, namely: polymerase chain reaction (PCR), real-time polymerase chain reaction (RT-PCR), loop-mediated isothermal amplification (LAMP), Luminex xMAP, random amplified polymorphic DNA (RAPD), amplified fragment length polymorphism (AFLP), and restriction fragment length polymorphism (RFLP), in addition to microsatellites. Molecular assays have comprehensively assisted in the diagnosis, treatment and epidemiological studies of parasitic diseases that affect people worldwide, helping to control parasitic disease mortality.
Contido em The journal of venomous animals and toxins including tropical diseases. Botucatu, SP. Vol. 17 n. 3 (2011), p. 239-248
Assunto Biologia molecular : Tecnica : Metodo
Doenças parasitárias
[en] Diagnosis
[en] Molecular epidemiology
[en] Molecular techniques
[en] Parasite infection
Origem Nacional
Tipo Artigo de periódico
URI http://hdl.handle.net/10183/77350
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