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Genetic similarity among strawberry cultivars assessed by RAPD and ISSR markers

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Genetic similarity among strawberry cultivars assessed by RAPD and ISSR markers

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Título Genetic similarity among strawberry cultivars assessed by RAPD and ISSR markers
Autor Morales, Rafael Gustavo Ferreira
Resende, Juliano Tadeu Vilela
Faria, Marcos Ventura
Andrade, Marcela Carvalho
Resende, Luciane Vilela
Delatorre, Carla Andrea
Silva, Paulo Roberto da
Abstract Most strawberry (Fragaria × ananassa Duchesne) cultivars used in Brazil are developed in other countries, it became clear the need to start the strawberry breeding program in the country. To start a breeding program is necessary the genetic characterization of the germplasm available. Molecular markers are important tools that can be used for this purpose. The objectives of the present study were to assess the genetic similarity among 11 strawberry cultivars using RAPD and ISSR molecular markers and to indicate the possible promising crosses. The DNA of the eleven strawberry cultivars was extracted and amplified by PCR with RAPD and ISSR primers. The DNA fragments were separated in agarose gel for the RAPD markers and in polyacrylamide gel for the ISSR markers. The genetic similarity matrix was estimated by the Jaccard coefficient. Based on this matrix, the cultivars were grouped using the UPGMA method. The dendogram generated by the RAPD markers distributed the cultivars in three groups while the ISSR markers generated two groups. There was no direct relationship between the marker groups when the two types of markers were compared. The grouping proposed by the ISSR markers was more coherent with the origin and the genealogy of the cultivars than that proposed by the RAPD markers, and it can be considered the most efficient method for the study of genetic divergence in strawberry. The most promising crosses, based on the genetic divergence estimated from the RAPD and ISSR molecular data were between the Tudla and Ventana and the Oso Grande and Ventana cultivars, respectively.
Contido em Scientia Agricola. Piracicaba. Vol. 68, n.6 (nov./dez. 2011), p. 665-670
Assunto Marcadores moleculares
Melhoramento vegetal
Morango
[en] Ananassa
[en] Fragaria
[en] Genetic divergence
[en] Molecular markers
[en] Plant breeding
Origem Nacional
Tipo Artigo de periódico
URI http://hdl.handle.net/10183/77916
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