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Micronuclei induced by reverse transcriptase inhibitors in mononucleated and binucleated cells as assessed by the cytokinesis-block micronucleus assay

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Micronuclei induced by reverse transcriptase inhibitors in mononucleated and binucleated cells as assessed by the cytokinesis-block micronucleus assay

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Título Micronuclei induced by reverse transcriptase inhibitors in mononucleated and binucleated cells as assessed by the cytokinesis-block micronucleus assay
Autor Lourenço, Eloir D.
Amaral, Viviane Souza do
Lehmann, Maurício
Dihl, Rafael Rodrigues
Schmitt, Virginia M.
Cunha, Kenya Silva
Reguly, Maria Luiza
Andrade, Heloisa Helena Rodrigues de
Abstract This study evaluated the clastogenic and/or aneugenic potential of three nucleoside reverse transcriptase inhibitors (zidovudine - AZT, lamivudine - 3TC and stavudine - d4T) using the cytokinesis-block micronucleus (CBMN) assay in human lymphocyte cultures. All three inhibitors produced a positive response when tested in binucleated cells. The genotoxicity of AZT and 3TC was restricted to binucleated cells since there was no significant increase in the frequency of micronuclei in mononucleated cells. This finding indicated that AZT and 3TC caused chromosomal breakage and that their genotoxicity was related to a clastogenic action. In addition to the positive response observed with d4T in binucleated cells, this drug also increased the frequency of micronuclei in mononucleated cells, indicating clastogenic and aneugenic actions. Since the structural differences between AZT and 3TC and AZT and d4T involve the 3’ position in the 2’-deoxyribonucleoside and in an unsaturated 2’,3’,dideoxyribose, respectively, we suggest that an unsaturated 2’, 3’, dideoxyribose is responsible for the clastogenic and aneugenic actions of d4T.
Contido em Genetics and molecular biology. Vol. 33, n. 4 (out./dez. 2010), p. 756-760
Assunto Estavudina
Lamivudina
Testes para micronúcleos
Zidovudina
[en] Lamivudine
[en] Micronucleus assay
[en] Stavudine
[en] Transcriptase inhibitors
[en] Zidovudine
Origem Nacional
Tipo Artigo de periódico
URI http://hdl.handle.net/10183/81189
Arquivos Descrição Formato
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