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On the applicability of SiO2/Al2O3/Nb2O5 and SiO2/Al2O3/TiO2 as a biocompatible platform for chloroperoxidase

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On the applicability of SiO2/Al2O3/Nb2O5 and SiO2/Al2O3/TiO2 as a biocompatible platform for chloroperoxidase

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Título On the applicability of SiO2/Al2O3/Nb2O5 and SiO2/Al2O3/TiO2 as a biocompatible platform for chloroperoxidase
Autor Ribeiro, Márcia Simões
Vasconcellos Júnior, Fábio Jorge de
Fonseca, Bruna Teixeira da
Souza, Flávia Carvalho de
Soares, Felipo Doval Rojas
Lima, Éder Cláudio
Cabral, Murilo Feitosa
Ribeiro, Emerson Schwingel
D'Elia, Eliane
Abstract In the present work, two mixed oxides, namely, SiO2/Al2O3/Nb2O5 and SiO2/Al2O3/TiO2 (designated as SiAlNb and SiAlTi, respectively), obtained using the sol–gel method were used to immobilize chloroperoxidase. Hydrogen peroxide was quantified using potassium hexacyanoferrate(II) as a redoxmediator and amperometric measurements at 0.0 V vs. Ag/AgCl/Cl (3 M). The SiAlTi biosensor presented higher sensitivity than the SiAlNb biosensor, however, the first one did not present a good response regarding time. The developed biosensor using the SiAlNb mixed oxide provided good signal levels, good linearity, good stability (retaining approximately 70% of its original response after 6 weeks of usage), a low detection limit (3 цM), good sensitivity, a suitable working range (from 4 to 19 цM), fast response and good repeatability. The recovery of the amperometric method for the detection of hydrogen peroxide in synthetic samples was approximately 100 ±2%, and for Listerine® Whitening Pre- Brush Rinse samples fortified with 1, 2 and 3% (v/v) of hydrogen peroxide, it was 100 ±3%.
Contido em Analytical Methods. Londres. Vol. 6, no. 2 (Jan. 2014), p. 521-528
Assunto Biosensores
Química analítica
Sol-gel
Origem Estrangeiro
Tipo Artigo de periódico
URI http://hdl.handle.net/10183/85929
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