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dc.contributor.authorKratz, Jadel Mullerpt_BR
dc.contributor.authorTeixeira, Marinapt_BR
dc.contributor.authorKoester, Leticia Schererpt_BR
dc.contributor.authorSimoes, Claudia Maria de Oliveirapt_BR
dc.date.accessioned2014-02-26T01:51:29Zpt_BR
dc.date.issued2011pt_BR
dc.identifier.issn0100-879Xpt_BR
dc.identifier.urihttp://hdl.handle.net/10183/87717pt_BR
dc.description.abstractThe Caco-2 cell line has been used as a model to predict the in vitro permeability of the human intestinal barrier. The predictive potential of the assay relies on an appropriate in-house validation of the method. The objective of the present study was to develop a single HPLC-UV method for the identification and quantitation of marker drugs and to determine the suitability of the Caco-2 cell permeability assay. A simple chromatographic method was developed for the simultaneous determination of both passively (propranolol, carbamazepine, acyclovir, and hydrochlorothiazide) and actively transported drugs (vinblastine and verapamil). Separation was achieved on a C18 column with step-gradient elution (acetonitrile and aqueous solution of ammonium acetate, pH 3.0) at a flow rate of 1.0 mL/min and UV detection at 275 nm during the total run time of 35 min. The method was validated and found to be specific, linear, precise, and accurate. This chromatographic system can be readily used on a routine basis and its utilization can be extended to other permeability models. The results obtained in the Caco-2 bi-directional transport experiments confirmed the validity of the assay, given that high and low permeability profiles were identified, and Pglycoprotein functionality was established.en
dc.format.mimetypeapplication/pdf
dc.language.isoengpt_BR
dc.relation.ispartofBrazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas. Ribeirão Preto. Vol. 44, n. 6 (2011), p. 531-537pt_BR
dc.rightsOpen Accessen
dc.subjectCaco-2en
dc.subjectCromatografia liquida de alta eficiencia (clae)pt_BR
dc.subjectEspectroscopia ultravioletapt_BR
dc.subjectHPLC-UVen
dc.subjectMarker drugsen
dc.subjectPermeabilidadept_BR
dc.subjectPermeabilityen
dc.titleAn hplc-uv method for the measurement of permeability of marker drugs in the caco-2 cell assaypt_BR
dc.typeArtigo de periódicopt_BR
dc.identifier.nrb000824648pt_BR
dc.type.originNacionalpt_BR


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