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A combined enrichment/polymerase chain reaction based method for the routine screening of streptococcus agalactiae in pregnant women

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A combined enrichment/polymerase chain reaction based method for the routine screening of streptococcus agalactiae in pregnant women

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Título A combined enrichment/polymerase chain reaction based method for the routine screening of streptococcus agalactiae in pregnant women
Autor Munari, Fernanda Mosena
Paris, Fernanda de
Salton, Gabrielle Dias
Lora, Priscila Schmidt
Giovanella, Patricia
Machado, Alice Beatriz Mombach Pinheiro
Laybauer, Letícia Saldanha
Oliveira, Kátia Ruschel Pilger de
Ferri, Cleusa
Silveira, João Lúcio dos Santos
Laurino, Claudia Cilene Fernandes Correia
Xavier, Ricardo Machado
Barth, Afonso Luis
Echeverrigaray, Sergio
Laurino, Jomar Pereira
Abstract Group B Streptococcus (GBS) is the most common cause of life-threatening infection in neonates. Guidelines from CDC recommend universal screening of pregnant women for rectovaginal GBS colonization. The objective of this study was to compare the performance of a combined enrichment/PCR based method targeting the atr gene in relation to culture using enrichment with selective broth medium (standard method) to identify the presence of GBS in pregnant women. Rectovaginal GBS samples from women at 36 weeks of pregnancy were obtained with a swab and analyzed by the two methods. A total of 89 samples were evaluated. The prevalence of positive results for GBS detection was considerable higher when assessed by the combined enrichment/PCR method than with the standard method (35.9% versus 22.5%, respectively). The results demonstrated that the use of selective enrichment broth followed by PCR targeting the atr gene is a highly sensitive, specific and accurate test for GBS screening in pregnant women, allowing the detection of the bacteria even in lightly colonized patients. This PCR methodology may provide a useful diagnostic tool for GBS detection and contributes for a more accurate and effective intrapartum antibiotic and lower newborn mortality and morbidity.
Contido em Brazilian journal of microbiology. São Paulo. Vol. 43, n. 1 (jan./mar. 2012), p. 253-260
Assunto Gestantes
Streptococcus agalactiae
[en] Accuracy
[en] atr gene
[en] Group B Streptococcus
[en] Screening test
[en] Streptococcus agalactiae
Origem Nacional
Tipo Artigo de periódico
URI http://hdl.handle.net/10183/87821
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