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The presence of sboA and spaS genes and antimicrobial peptides subtilosin A and subtilin among Bacillus strains of the Amazon basin

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The presence of sboA and spaS genes and antimicrobial peptides subtilosin A and subtilin among Bacillus strains of the Amazon basin

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Título The presence of sboA and spaS genes and antimicrobial peptides subtilosin A and subtilin among Bacillus strains of the Amazon basin
Autor Velho, Renata Voltolini
Basso, Ana Paula
Segalin, Jéferson
Medina, Luis Fernando da Costa
Brandelli, Adriano
Abstract This report demonstrates the usefulness of PCR for the genes spaS and sboA as a means of identifying Bacillus strains with a potential to produce subtilin and subtilosin A. One collection strain and five Bacillus spp. isolated from aquatic environments in the Amazon basin were screened by PCR using primers for sboA and spaS designed specifically for this study. The sequences of the PCR products showed elevated homology with previously described spaS and sboA genes. Antimicrobial peptides were isolated from culture supernatants and analyzed by mass spectrometry. For all samples, the mass spectra revealed clusters with peaks at m/z 3300-3500 Da, corresponding to subtilosin A, subtilin and isoforms of these peptides. These results suggest that the antimicrobial activity of these strains may be associated with the production of subtilosin A and/or subtilin. The PCR used here was efficient in identifying novel Bacillus strains with the essential genes for producing subtilosin A and subtilin.
Contido em Genetics and molecular biology. Vol. 36, n. 1 (Mar. 2013), p. 101-104
Assunto Bacillus
Bacilos
Bacteriocina
Polimerase : Reação em cadeia
Reação em cadeia da polimerase
[en] Bacillus
[en] bacteriocin
[en] polymerase chain reaction
[en] subtilin
[en] subtilosin A
Origem Nacional
Tipo Artigo de periódico
URI http://hdl.handle.net/10183/87938
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