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dc.contributor.authorTondo, Eduardo Cesarpt_BR
dc.contributor.authorMachado, Taís Raquel Marconpt_BR
dc.contributor.authorMalheiros, Patricia da Silvapt_BR
dc.contributor.authorPadrão, Débora Krügerpt_BR
dc.contributor.authorCarvalho, Ana Lyl Oliveira dept_BR
dc.contributor.authorBrandelli, Alexandrept_BR
dc.date.accessioned2014-02-28T01:50:56Zpt_BR
dc.date.issued2010pt_BR
dc.identifier.issn1517-8382pt_BR
dc.identifier.urihttp://hdl.handle.net/10183/88000pt_BR
dc.description.abstractThe adhesion of Salmonella (S) strains to stainless steel and polyethylene and their inactivation by biocides used in food industry was investigated. Coupons of stainless steel and polyethylene were immersed in bacterial suspensions of S. Enteritidis,S.Typhimurium, and S. Bredeney during 15, 30, and 60 minutes, and submitted to different concentrations of peracetic acid (PAA), sodium hypochlorite (NaOCl), and quaternary ammonium(Quat) sanitizers. Hydrophobicity of the surfaces was evaluated by contact angle measurements using the sessile drop method and bacterial adhesion was accompanied through bacterial counts and scanning electron microscopy(SEM). Results indicated that the three serovars of Salmonella presented similar adhesion to both materials (5.0 to 6.5 log cfu cm-2). The time of exposure did not influence the counts of adhered cells on both surfaces, however SEM revealed larger clusters of S. Enteritidis on both materials, not found for the other serovars. S. Enteritidis presented lower sessile drop angle on polyethylene, indicating hydrophilic properties of this material. The biocides were not able to inactivate all the microorganisms adhered on both surfaces. At least 1 log cfu cm-2 of all serovars tested remained viable after the exposure to different biocide concentrations. In general, higher counts of survivors were observed on polyethylene disinfected with different concentrations of biocides. S. Bredeney e S. Typhimurium were more resistant than S. Enteritidis to PAA, whilst S. Enteritidis presented smaller reduction rates to NaOCl.This last biocide was able to reduce Salmonella counts in approximately 3.0 to 4.0 log cm-2. When adhered to polyethylene, the serovars S. Typhimurium and S. Enteritidis were more resistant to Quat than S. Bredeney in all concentrations tested, and the numbers of S. Enteritidis remained almost unaltered. On stainless steel disinfected by Quat, S. Bredeney presented high numbers of survivors.en
dc.format.mimetypeapplication/pdfpt_BR
dc.language.isoengpt_BR
dc.relation.ispartofBrazilian journal of microbiology. São Paulo. Vol. 41, no. 4 (oct./dez. 2010), p. 1027-1037pt_BR
dc.rightsOpen Accessen
dc.subjectSalmonellaen
dc.subjectSalmonellapt_BR
dc.subjectAdesãopt_BR
dc.subjectadhesionen
dc.subjectbiocidesen
dc.subjectAço inoxidávelpt_BR
dc.subjectBiocidaspt_BR
dc.subjectstainless steelen
dc.subjectPolietilenopt_BR
dc.subjectpolyethyleneen
dc.titleAdhesion and biocides inactivation of Salmonella on stainless steel and polyethylenept_BR
dc.typeArtigo de periódicopt_BR
dc.identifier.nrb000903126pt_BR
dc.type.originNacionalpt_BR


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