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Aging and its impact onthe quality of grafts : an experimental study in rats livers

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Aging and its impact onthe quality of grafts : an experimental study in rats livers

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Título Aging and its impact onthe quality of grafts : an experimental study in rats livers
Outro título O impacto do envelhecimento na qualidade do enxerto : estudo experimental em fígados de ratos
Autor Mota, Stela Maria
Gasperin, Glauber
Cerski, Carlos Thadeu Schmidt
Oliveira, Jarbas Rodrigues de
Álvares-da-Silva, Mário Reis
Abstract There are consistent clues of worse results with grafts from older donors, especially in hepatitis C. University of Wisconsin (UW) solution is adopted for liver preservation, but other solutions are being studied, as fructose-1,6-bisphosphate (FBP). Objective – To determinate the impact of aging of the donor on the cold ischemia injury in rat livers and compare UW and FBP. Methods – Twenty male Wistar rats were studied - 10, aging 20 weeks: 5 to preservation with UW (C-UW) and 5, FBP (C-FBP); and other 10, aging 50 weeks: 5 to UW (E-UW) and 5 to FBP (E-FBP). Rats were anesthesized, submitted to hepatectomy, and graft was kept under 2-4ºC for 8 hours. Liquid samples were taken at 2, 4, 6, and 8 hours, to determine AST and LDH. At the end, in the liver tissue thiobarbituric acid reactive substances and catalase were determined. Results - Average liver weight was similar between controls and the others (12.5 g ± 1.8 and 13.4 g ± 2.0). AST and LDH levels were higher in C-UW group (P<0.05). In the older group, there was a difference between UW and FBP preserved livers related to LDH, but not to AST. Thiobarbituric acid reactive substances were superior in control group than in the older one (P = 0.001). Catalase activity was similar between these groups (P = 0.11), but it was superior in UW preserved animals (P = 0.02). Conclusion - Livers from older rats were similar to the controls regarding cold ischemia injury in FBP group. Surprisingly, with UW solution there was less cold ischemia injury in the elderly group. When comparing both solutions, FBP provided significantly more protection than UW in the controls. There was a trend to FBP to being better than UW in the elderly group. Further studies with liver from older donors and ischemia and reperfusion are needed.
Contido em Arquivos de gastroenterologia = Archives of gastroenterology. São Paulo. Vol. 47, n. 3 (jul.-set. 2010), p. 297-300
Assunto Adenosina
Alopurinol
Animais
Criopreservação
Fatores etários
Fígado
Frutosedifosfatos
Glutationa
Insulina
Masculino
Métodos
Presevação de órgãos
Rafinose
Ratos Wistar
Soluções para preservação de órgãos
[en] Aging
[en] Liver transplantation
[en] Rats
[en] Tissue donors
Origem Nacional
Tipo Artigo de periódico
URI http://hdl.handle.net/10183/88209
Arquivos Descrição Formato
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