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N-acetylcysteine improves antitumoural response of Interferon alpha by NF-kB downregulation in liver cancer cells

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N-acetylcysteine improves antitumoural response of Interferon alpha by NF-kB downregulation in liver cancer cells

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Título N-acetylcysteine improves antitumoural response of Interferon alpha by NF-kB downregulation in liver cancer cells
Autor Kretzmann, Nelson Alexandre
Chiela, Eduardo Cremonese Filippi
Matte, Ursula da Silveira
Marroni, Norma Anair Possa
Marroni, Claudio Augusto
Abstract Background: Liver cancer is one of the most common malignancies in the world and at the moment, there is no drug intervention effective for the treatment of liver tumours. Investigate the effect of N-acetylcysteine (NAC), which has been studied for its antitumoural properties, on the toxicity of hepatocarcinoma (HCC) cells in vitro when used with the drug interferon alpha-2A (IFN), which is used clinically to treat HCC. Results: NAC, IFN and NAC plus IFN reduced cell viability, as determined by MTT assay. More importantly, NAC potentiates the cytotoxic effect of IFN, with the best response achieved with 10 mM of NAC and 2.5 x 104 of IFN. These results were confirmed by Annexin/PI staining through flow cytometry and morphologic analyses. Co-treatment reduced the expression of the nuclear transcription factor kappa-B (NF-kB). In a similar way to NAC, RNAi against p65 potentiated the toxic effect of IFN, suggesting that, indeed, NAC may be enhancing the effect of IFN through inhibition of NF-kB. Conclusions: Our results support the notion that NAC may be an important drug for the treatment of liver tumours as primary or adjuvant therapy. IFN has a limited clinical response, and therefore, the anti-proliferative properties of NAC in the liver should be explored further as an alternative for non-responders to IFN treatment.
Contido em Comparative hepatology. London. Vol. 11 (2012), 9 p.
Assunto Acetilcisteína
Interferon alfa
Neoplasias hepáticas
[en] Interferon alpha
[en] Liver cancer cells
[en] N-acetylcysteine
[en] NF-kB
Origem Estrangeiro
Tipo Artigo de periódico
URI http://hdl.handle.net/10183/89710
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