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Methotrexate diethyl ester-loaded lipid-core nanocapsules in aqueous solution increased antineoplastic effects in resistant breast cancer cell line

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Methotrexate diethyl ester-loaded lipid-core nanocapsules in aqueous solution increased antineoplastic effects in resistant breast cancer cell line

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Título Methotrexate diethyl ester-loaded lipid-core nanocapsules in aqueous solution increased antineoplastic effects in resistant breast cancer cell line
Autor Yurgel, Virgina Campello
Oliveira, Catiúscia Padilha de
Begnini, Karine Rech
Schultze, Eduarda
Thurow, Helena Strelow
Leon, Priscila Marques Moura de
Dellagostin, Odir Antônio
Campos, Vinicius Farias
Beck, Ruy Carlos Ruver
Guterres, Silvia Stanisçuaski
Collares, Tiago Veiras
Pohlmann, Adriana Raffin
Seixas, Fabiana Kömmling
Abstract Breast cancer is the most frequent cancer affecting women. Methotrexate (MTX) is an antimetabolic drug that remains important in the treatment of breast cancer. Its efficacy is compromised by resistance in cancer cells that occurs through a variety of mechanisms. This study evaluated apoptotic cell death and cell cycle arrest induced by an MTX derivative (MTX diethyl ester [MTX(OEt)2]) and MTX(OEt)2-loaded lipid-core nanocapsules in two MTX-resistant breast adenocarcinoma cell lines, MCF-7 and MDA-MB-231. The formulations prepared presented adequate granulometric profile. The treatment responses were evaluated through flow cytometry. Relying on the mechanism of resistance, we observed different responses between cell lines. For MCF-7 cells, MTX(OEt)2 solution and MTX(OEt)2-loaded lipid-core nanocapsules presented significantly higher apoptotic rates than untreated cells and cells incubated with unloaded lipid-core nanocapsules. For MDA-MB-231 cells, MTX(OEt)2-loaded lipid-core nanocapsules were significantly more efficient in inducing apoptosis than the solution of the free drug. S-phase cell cycle arrest was induced only by MTX(OEt)2 solution. The drug nanoencapsulation improved apoptosis induction for the cell line that presents MTX resistance by lack of transport receptors.
Contido em International Journal of Nanomedicine. Macclesfield, UK. Vol. 9, no. 1 (2014), p. 1583-1591
Assunto Metotrexato
Neoplasias da mama
[en] Apoptotic cell death
[en] Cell cycle arrest
[en] Drug delivery
[en] Drug targeting
[en] MTX derivative
[en] Nanocarriers
[en] Resistance
Origem Estrangeiro
Tipo Artigo de periódico
URI http://hdl.handle.net/10183/97020
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