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dc.contributor.authorFernandes, Kristianne Porta Santospt_BR
dc.contributor.authorBussadori, Sandra Kalilpt_BR
dc.contributor.authorMarques, Márcia Martinspt_BR
dc.contributor.authorWadt, Nilsa Sumie Yamashitapt_BR
dc.contributor.authorBach, Ernapt_BR
dc.contributor.authorMartins, Manoela Dominguespt_BR
dc.date.accessioned2016-10-26T02:19:38Zpt_BR
dc.date.issued2010pt_BR
dc.identifier.issn1677-3217pt_BR
dc.identifier.urihttp://hdl.handle.net/10183/149245pt_BR
dc.description.abstractAim: The aims of this study were to evaluate the wound healing potential in vivo and the cytotoxic effects in vitro of Psidium guajava (Myrtaceae) leaf extract and commonly used corticosteroids. Methods: The healing effect was studied in vivo by the clinical and histological evaluation of traumatic lesions in the oral mucosa of rats treated with these substances. Each rat received 2 daily applications of the medicine tested and the animals were sacrificed after 1, 3, 5, 7 and 14 days. Tissue sections stained with hematoxylin & eosin were analyzed. The histological evaluation involved a 5-point scoring system based on the degree of healing, ranging from 1 (total repair of epithelium and connective tissue) to 5 (epithelial ulcer and acute inflammatory infiltrate). The Kruskal-Wallis test was used for statistical analysis of the histological scores. For the in vitro toxicity assay, each substance was applied to mucosa fibroblast cell cultures in conditioned media. The media were conditioned by placing the substances in contact with fresh culture medium for 24 h. The cytotoxicity analysis was performed using the MTT assay. Data were analyzed statistically by ANOVA and Tukey’s test at 5% significance level. Results: In vitro, the guava extract caused a decrease in the cell viability and growth when compared to the control and corticosteroids. In vivo, guava extract caused accelerated wound healing from the 3rd day, whereas the corticosteroids delayed tissue repair and were associated with bacterial surface colonization, the presence of micro-abscesses and intense inflammatory infiltrate in the submucosa. Conclusions: Although in a short-term cytotoxicity analysis, the guava extract reduced the cell population in vitro, while in vivo, the extract accelerated wound healing.en
dc.format.mimetypeapplication/pdf
dc.language.isoengpt_BR
dc.relation.ispartofBrazilian journal of oral sciences. Piracicaba. Vol. 9, no. 4 (Oct./Dec. 2010), p. 449-454pt_BR
dc.rightsOpen Accessen
dc.subjectPsidium guajavaen
dc.subjectFitoterapiapt_BR
dc.subjectCitotoxicidadept_BR
dc.subjectWound healingen
dc.subjectCell cultureen
dc.subjectGuava leavesen
dc.subjectCytotoxicityen
dc.titleHealing and cytotoxic effects of Psidium guajava (Myrtaceae) leaf extractspt_BR
dc.typeArtigo de periódicopt_BR
dc.identifier.nrb000709179pt_BR
dc.type.originNacionalpt_BR


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