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dc.contributor.authorNóbrega, Letícia Maria Menezespt_BR
dc.contributor.authorMontagner, Franciscopt_BR
dc.contributor.authorRibeiro, Adriana Costapt_BR
dc.contributor.authorMayer, Marcia Pinto Alvespt_BR
dc.contributor.authorGomes, Brenda Paula Figueiredo de Almeidapt_BR
dc.date.accessioned2017-07-25T02:30:07Zpt_BR
dc.date.issued2016pt_BR
dc.identifier.issn1806-8324pt_BR
dc.identifier.urihttp://hdl.handle.net/10183/164326pt_BR
dc.description.abstractThe aim of this study was to explore the bacterial diversity of 10 root canals with acute apical abscess using clonal analysis. Samples were collected from 10 patients and submitted to bacterial DNA isolation, 16S rRNA gene amplification, cloning, and sequencing. A bacterial genomic library was constructed and bacterial diversity was estimated. The mean number of taxa per canal was 15, ranging from 11 to 21. A total of 689 clones were analyzed and 76 phylotypes identified, of which 47 (61.84%) were different species and 29 (38.15%) were taxa reported as yet-uncultivable or as yet-uncharacterized species. Prevotella spp., Fusobacterium nucleatum, Filifactor alocis, and Peptostreptococcus stomatis were the most frequently detected species, followed by Dialister invisus, Phocaeicola abscessus, the uncharacterized Lachnospiraceae oral clone, Porphyromonas spp., and Parvimonas micra. Eight phyla were detected and the most frequently identified taxa belonged to the phylum Firmicutes (43.5%), followed by Bacteroidetes (22.5%) and Proteobacteria (13.2%). No species was detected in all studied samples and some species were identified in only one case. It was concluded that acute primary endodontic infection is characterized by wide bacterial diversity and a high intersubject variability was observed. Anaerobic Gram-negative bacteria belonging to the phylum Firmicutes, followed by Bacteroidetes, were the most frequently detected microorganisms.en
dc.format.mimetypeapplication/pdfpt_BR
dc.language.isoengpt_BR
dc.relation.ispartofBrazilian oral research. São Paulo. Vol. 30, no. 1 (Oct./Dec. 2016), p. 1-9pt_BR
dc.rightsOpen Accessen
dc.subjectEndodontiapt_BR
dc.subjectEndodonticsen
dc.subjectMicrobiologyen
dc.subjectBactériaspt_BR
dc.subjectMicrobiologiapt_BR
dc.subjectCloningen
dc.subjectMolecularen
dc.titleBacterial diversity of symptomatic primary endodontic infection by clonal analysispt_BR
dc.typeArtigo de periódicopt_BR
dc.identifier.nrb001017475pt_BR
dc.type.originNacionalpt_BR


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