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dc.contributor.authorTirloni, Lucaspt_BR
dc.contributor.authorKim, Tae Kwonpt_BR
dc.contributor.authorPinto, Antonio Frederico Michelpt_BR
dc.contributor.authorYates III, John R.pt_BR
dc.contributor.authorVaz Junior, Itabajara da Silvapt_BR
dc.contributor.authorMulenga, Albertpt_BR
dc.date.accessioned2018-03-14T02:38:18Zpt_BR
dc.date.issued2017pt_BR
dc.identifier.issn2235-2988pt_BR
dc.identifier.urihttp://hdl.handle.net/10183/173336pt_BR
dc.description.abstractUnderstanding the molecular basis of how ticks adapt to feed on different animal hosts is central to understanding tick and tick-borne disease (TBD) epidemiology. There is evidence that ticks differentially express specific sets of genes when stimulated to start feeding. This study was initiated to investigate if ticks such as Ixodes scapularis and Amblyomma americanum that are adapted to feed on multiple hosts utilized the same sets of proteins to prepare for feeding. We exposed I. scapularis and A. americanum to feeding stimuli of different hosts (rabbit, human, and dog) by keeping unfed adult ticks enclosed in a perforated microfuge in close contact with host skin, but not allowing ticks to attach on host. Our data suggest that ticks of the same species differentially express tick saliva proteins (TSPs) when stimulated to start feeding on different hosts. SDS-PAGE and silver staining analysis revealed unique electrophoretic profiles in saliva of I. scapularis and A. americanum that were stimulated to feed on different hosts: rabbit, human, and dog. LC-MS/MS sequencing and pairwise analysis demonstrated that I. scapularis and A. americanum ticks expressed unique protein profiles in their saliva when stimulated to start feeding on different hosts: rabbit, dog, or human. Specifically, our data revealed TSPs that were unique to each treatment and those that were shared between treatments. Overall, we identified a total of 276 and 340 non-redundant I. scapularis and A. americanum TSPs, which we have classified into 28 functional classes including: secreted conserved proteins (unknown functions), proteinase inhibitors, lipocalins, extracellularmatrix/cell adhesion, heme/ironmetabolism, signal transduction and immunity-related proteins being the most predominant in saliva of unfed ticks. With exception of research on vaccines against Rhipicephalus microplus, which its natural host, cattle, research on vaccine against other ticks relies feeding ticks on laboratory animals. Data here suggest that relying on lab animal tick feeding data to select target antigens could result in prioritizing irrelevant anti-tick vaccine targets that are expressed when ticks feed on laboratory animals. This study provides the platform that could be utilized to identify relevant target anti-tick vaccine antigens, and will facilitate early stage tick feeding research.en
dc.format.mimetypeapplication/pdf
dc.language.isoengpt_BR
dc.relation.ispartofFrontiers in cellular and infection microbiology. Lausanne. Vol. 7, Article 517, (Dec. 2017), 13 p.pt_BR
dc.rightsOpen Accessen
dc.subjectTicken
dc.subjectCarrapatopt_BR
dc.subjectIxodes scapularispt_BR
dc.subjectSalivaen
dc.subjectProteomicen
dc.subjectAmblyomma americanumpt_BR
dc.subjectTick-host relationshipen
dc.subjectGenética molecularpt_BR
dc.subjectSalivapt_BR
dc.subjectHost adaptationen
dc.subjectProteômicapt_BR
dc.subjectProteínaspt_BR
dc.subjectHospedeiropt_BR
dc.titleTick-host range adaptation : changes in protein profiles in unfed adult Ixodes scapularis and Amblyomma americanum saliva stimulated to feed on different hostspt_BR
dc.typeArtigo de periódicopt_BR
dc.identifier.nrb001056541pt_BR
dc.type.originEstrangeiropt_BR


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