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dc.contributor.authorAntonow, Michelli Barcelospt_BR
dc.contributor.authorFranco, Camilapt_BR
dc.contributor.authorPrado, Willian Andradept_BR
dc.contributor.authorBeckenkamp, Alinept_BR
dc.contributor.authorSilveira, Gustavo Pozzapt_BR
dc.contributor.authorBuffon, Andreiapt_BR
dc.contributor.authorGuterres, Silvia Stanisçuaskipt_BR
dc.contributor.authorPohlmann, Adriana Raffinpt_BR
dc.date.accessioned2018-09-26T02:33:35Zpt_BR
dc.date.issued2018pt_BR
dc.identifier.issn2079-4991pt_BR
dc.identifier.urihttp://hdl.handle.net/10183/182662pt_BR
dc.description.abstractDoxorubicin (Dox) clinical use is limited by dose-related cardiomyopathy, becoming more prevalent with increasing cumulative doses. Previously, we developed Dox-loaded lipid-core nanocapsules (Dox-LNC) and, in this study, we hypothesized that self-assembling and interfacial reactions could be used to obtain arginylglycylaspartic acid (RGD)-surface-functionalized-Dox-LNC, which could target tumoral cells overexpressing v 3 integrin. Human breast adenocarcinoma cell line (MCF-7) and human glioblastoma astrocytoma (U87MG) expressing different levels of v 3 integrin were studied. RGD-functionalized Dox-LNC were prepared with Dox at 100 and 500 mg mL􀀀1 (RGD-MCMN (Dox100) and RGD-MCMN (Dox500)). Blank formulation (RGD-MCMN) had z-average diameter of 162 6 nm, polydispersity index of 0.11 0.04, zeta potential of +13.2 1.9 mV and (6.2 1.1) 1011 particles mL􀀀1, while RGD-MCMN (Dox100) and RGD-MCMN (Dox500) showed respectively 146 20 and 215 25 nm, 0.10 0.01 and 0.09 0.03, +13.8 2.3 and +16.4 1.5 mV and (6.9 0.6) 1011 and (6.1 1.0) 1011 particles mL􀀀1. RGD complexation was 7.73 104 molecules per nanocapsule and Dox loading were 1.51 104 and 7.64 104 molecules per nanocapsule, respectively. RGD-functionalized nanocapsules had an improved uptake capacity by U87MG cells. Pareto chart showed that the cell viability was mainly affected by the Dox concentration and the period of treatment in both MCF-7 and U87MG. The influence of RGD-functionalization on cell viability was a determinant factor exclusively to U87MG.en
dc.format.mimetypeapplication/pdfpt_BR
dc.language.isoengpt_BR
dc.relation.ispartofNanomaterials. Suíça. Vol. 8, no. 1 (2018), p. 1-18pt_BR
dc.rightsOpen Accessen
dc.subjectLipid-core nanocapsulesen
dc.subjectNanocápsulas de núcleo lipídicopt_BR
dc.subjectCanceren
dc.subjectCâncerpt_BR
dc.subjectDoxorrubicinapt_BR
dc.subjectRGDen
dc.subjectDoxorubicinen
dc.subjectActive drug targetingen
dc.subjectSurface-functionalizationen
dc.titleArginylglycylaspartic Acid-Surface-Functionalized Doxorubicin-Loaded Lipid-Core nanocapsules as a strategy to target Alpha(V) Beta(3) integrin expressed on tumor cellspt_BR
dc.typeArtigo de periódicopt_BR
dc.identifier.nrb001076172pt_BR
dc.type.originEstrangeiropt_BR


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