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dc.contributor.authorLugo, Ana Ilda Ayalapt_BR
dc.contributor.authorTavares, Angela Maria Vicentept_BR
dc.contributor.authorPaz, Ana Helena da Rosapt_BR
dc.contributor.authorAlegretti, Ana Paulapt_BR
dc.contributor.authorMiquelito, Ludmila do Valept_BR
dc.contributor.authorBock, Hugopt_BR
dc.contributor.authorGiugliani, Robertopt_BR
dc.contributor.authorClausell, Nadine Oliveirapt_BR
dc.contributor.authorCirne Lima, Elizabeth Obinopt_BR
dc.contributor.authorRohde, Luis Eduardo Paimpt_BR
dc.date.accessioned2019-03-21T02:29:30Zpt_BR
dc.date.issued2011pt_BR
dc.identifier.issn0963-6897pt_BR
dc.identifier.urihttp://hdl.handle.net/10183/189487pt_BR
dc.description.abstractThe aim of this study was to investigate the effect of aging and timing of left ventricular ischemic injury on the availability and functionality of stem cells. We studied young and aged male inbred Lewis rats that were used as donors of bone marrow mononuclear cells (BM-MNCs), divided in four experimental groups: controls, sham operated, 48 h post-myocardial infarction (MI), and 28 days post-MI. In vitro studies included flow cytometry analysis, hematopoietic colony-forming capacity, and invasion assays of migration capacity. BM-MNCs from these groups were transplanted in female rats after MI induction. Late engraftment was evaluated by real-time PCR of the SRY chromosome. Percentage of CD34+/CD45+low cells was similar among different experimental groups in young rats, but was significantly higher in aged animals (p < 0.001), particularly 28 days post-MI. KDR+/CD34+ cells were increased 48 h after MI and decreased 28 days post-MI in young animals, while they were profoundly reduced in the aged group (p < 0.001). Triple staining for CD44+/CD29+/CD71+ cells was similar in different groups of aged rats, but we observed an intense increase 48 h post-MI in young animals. Colony-forming units and cytokine-induced migration were significantly attenuated 28 days after the MI. Late engraftment in infarcted transplanted female hearts was present, but considerably heterogeneous. Finally, recovery of left ventricular systolic function in transplanted female recipients was significantly influenced by donors’ BM-MNCs groups (p < 0.01). We have demonstrated that aging and timing of myocardial injury are factors that may act synergistically in determining stem cell availability and function. Such interaction should be considered when planning new cell therapy strategies for acute and chronic ischemic heart disease in the clinical arena.en
dc.format.mimetypeapplication/pdfpt_BR
dc.language.isoengpt_BR
dc.relation.ispartofCell transplantation. Elmsford. Vol. 20, no. 3 (2011), p. 407-419.pt_BR
dc.rightsOpen Accessen
dc.subjectTerapia tecidualpt_BR
dc.subjectCell therapyen
dc.subjectInfarto do miocárdiopt_BR
dc.subjectAgingen
dc.subjectMyocardial infarction (MI)en
dc.subjectEnvelhecimentopt_BR
dc.subjectModelos animais de doençaspt_BR
dc.titleAge-dependent availability and functionality of bone marrow stem cells in an experimental model of acute and chronic myocardial infarctionpt_BR
dc.typeArtigo de periódicopt_BR
dc.identifier.nrb000784457pt_BR
dc.type.originEstrangeiropt_BR


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