Navegação Ciências Biológicas por Autor "Zaha, Arnaldo"
Resultados 1-20 de 36
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Antigen B from Echinococcus granulosus enters mammalian cells by endocytic pathways
Silva, Edileuza Danieli da; Cancela, Martín; Monteiro, Karina Mariante; Ferreira, Henrique Bunselmeyer; Zaha, Arnaldo (2018) [Artigo de periódico]Background Cystic hydatid disease is a zoonosis caused by the larval stage (hydatid) of Echinococcus granulosus (Cestoda, Taeniidae). The hydatid develops in the viscera of intermediate host as a unilocular structure filled ... -
Breeding systems in Echinococcus granulosus (Cestoda; Taeniidae) : selfing or outcrossing?
Haag, Karen Luisa; Araujo, Aldo Mellender de; Gottstein, Bruno; Siles-Lucas, M.; Thompson, R. C. A.; Zaha, Arnaldo (1999) [Artigo de periódico]We used the PCR±SSCP method followed by sequencing in order to assess the genetic variability of coding and noncoding parts of the genome of Echinococcus granulosus (Cestoda; Taeniidae) and to test whether or not the ... -
Cestode strobilation: prediction of developmental genes and pathways
Paludo, Gabriela Prado; Thompson, Claudia Elizabeth; Miyamoto, Kendi Nishino; Guedes, Rafael Lucas Muniz; Zaha, Arnaldo; Vasconcelos, Ana Tereza Ribeiro de; Cancela, Martín; Ferreira, Henrique Bunselmeyer (2020) [Artigo de periódico]Background: Cestoda is a class of endoparasitic worms in the flatworm phylum (Platyhelminthes). During the course of their evolution cestodes have evolved some interesting aspects, such as their increased reproductive ... -
Characterization of mutations causing CYP21A2 deficiency in brazilian and portuguese populations
Prado, Mayara Jorgens; Singh, Shripriya; Braun, Rodrigo Ligabue; Meneghetti, Bruna Valandro; Serrano, Thaiane Rispoli; Kopacek, Cristiane; Monteiro, Karina Mariante; Zaha, Arnaldo; Rossetti, Maria Lucia Rosa; Pandey, Amit V. (2022) [Artigo de periódico]Deficiency of 21-hydroxylase enzyme (CYP21A2) represents 90% of cases in congenital adrenal hyperplasia (CAH), an autosomal recessive disease caused by defects in cortisol biosynthesis. Computational prediction and functional ... -
Cloning and characterization of Echinococcus granulosus (Cestode) EgactI and EgactII actin gene promoters and their functional analysis in the NIH3T3 mouse cell line
Gimba, Etel Rodrigues Pereira; Chemale, Gustavo; Farias, Sandra Estrazulas; Zaha, Arnaldo (2000) [Artigo de periódico]We report here for the first time the structure and function of a promoter from a cestode. The ability of DNA fragments respectively encompassing the 935-bp and 524-bp regions upstream from the ATG codon from the EgactI ... -
Colorimetric microwell plate reverse-hybridization assay for Mycobacterium tuberculosis detection
Michelon, Candice Tosi; Rosso, Franciele; Schmid, Karen Barros; Sperhacke, Rosa Dea; Oliveira, Martha Maria; Kritski, Afrânio Lineu; Rezende Junior, Leonides; Costa, Elis Regina Dalla; Ribeiro, Andrezza Wolowski; Verza, Mirela; Cafrune, Patricia Izquierdo; Silva, Marcia Susana Nunes; Kühleis, Daniele Chaves; Zaha, Arnaldo; Rossetti, Maria Lucia Rosa (2011) [Artigo de periódico]Direct smear examination using Ziehl-Neelsen staining for pulmonary tuberculosis (PTB) diagnosis is inexpensive and easy to use, but has the major limitation of low sensitivity. Rapid molecular methods are becoming more ... -
Comparative analysis of the 14-3-3 gene and its expression in Echinococcus granulosus and Echinococcus multilocularis metacestodes
Siles-Lucas, M.; Nunes, Claudia Paiva; Zaha, Arnaldo (2001) [Artigo de periódico]It was suggested that the unlimited proliferative capacity of the Echinococcus multilocularis metacestode may be related to overproduction of the 14-3-3 protein. As is known, the proliferative capacities of E. granulosus ... -
Comparative proteomic analysis of pathogenic and non-pathogenic strains from the swine pathogen Mycoplasma hyopneumoniae
Pinto, Paulo Marcos; Klein, Catia Silene; Zaha, Arnaldo; Ferreira, Henrique Bunselmeyer (2009) [Artigo de periódico]Background: Mycoplasma hyopneumoniae is a highly infectious swine pathogen and is the causative agent of enzootic pneumonia (EP). Following the previous report of a proteomic survey of the pathogenic 7448 strain of swine ... -
Detection of rifampin-resistant genotypes in Mycobacterium tuberculosis by reverse hybridization assay
Maschmann, Raquel de Abreu; Verza, Mirela; Silva, Marcia Susana Nunes; Sperhacke, Rosa Dea; Ribeiro, Marta Osório; Suffys, Philip Noel; Gomes, Harrison Magdinier; Tortoli, Enrico; Marcelli, Fiorella; Zaha, Arnaldo; Rossetti, Maria Lucia Rosa (2011) [Artigo de periódico]We used a colorimetric reverse dot blot hybridization (CRDH) assay to detect the presence of mutations in a specific region of the rpoB gene, associated with rifampin (RIF) resistance, in a panel of 156 DNAs extracted from ... -
Differential domains and endoproteolytic processing in dominant surface proteins of unknown function from Mycoplasma hyopneumoniae and Mycoplasma flocculare
Santos, Priscila Souza dos; Paes, Jéssica Andrade; Machado, Lais Del Prá Netto; Paludo, Gabriela Prado; Zaha, Arnaldo; Ferreira, Henrique Bunselmeyer (2023) [Artigo de periódico]Mycoplasma hyopneumoniae causes porcine enzootic pneumonia (PEP), a chronic respiratory disease that leads to severe economic losses in the pig industry. Swine infection and PEP development depend on the adhesion of the ... -
Echinococcus granulosus antigen b structure : subunit composition and oligomeric states
Monteiro, Karina Mariante; Drumond, Fernanda; Cardoso, Mateus Borba; Follmer, Cristian; Silveira, Nádya Pesce da; Vargas, Daiani Machado de; Kitajima, Elliot Watanabe; Zaha, Arnaldo; Ferreira, Henrique Bunselmeyer (2012) [Artigo de periódico] -
Evaluation of real-time PCR of patient pleural effusion for diagnosis of tuberculosis
Rosso, Franciele; Michelon, Candice Tosi; Sperhacke, Rosa Dea; Verza, Mirela; Olival, Liliane; Conde, Marcus Barreto; Guerra, Renata L.; Zaha, Arnaldo; Rossetti, Maria Lucia Rosa (2011) [Artigo de periódico]Background: Pleural tuberculosis (TB) diagnosis often requires invasive procedures such as pleural biopsy. The aim of this study was to evaluate the role of real-time polymerase chain reaction (PCR) for the IS6110 sequence ... -
Evolutionary fates of universal stress protein paralogs in Platyhelminthes
Espínola, Sérgio Martin; Cancela, Martín; Corrêa, Lauís Brisolara; Zaha, Arnaldo (2018) [Artigo de periódico]Background Universal stress proteins (USPs) are present in all domains of life. Their expression is upregulated in response to a large variety of stress conditions. The functional diversity found in this protein family, ... -
Genes involved in cell division in mycoplasmas
Alarcón, Frank; Vasconcelos, Ana Tereza Ribeiro de; Yim, Lucia; Zaha, Arnaldo (2007) [Artigo de periódico]Bacterial cell division has been studied mainly in model systems such as Escherichia coli and Bacillus subtilis, where it is described as a complex process with the participation of a group of proteins which assemble into ... -
High polymorphism in genes encoding antigen B from human infecting strains of Echinococcus granulosus
Kamenetzky, Laura; Muzulin, P. M.; Gutierrez, Ariana M.; Angel, S.O.; Zaha, Arnaldo; Guarnera, Eduardo A.; Rosenzvit, Mara Cecília (2005) [Artigo de periódico]Echinococcus granulosus antigen B (AgB) is encoded by a gene family and is involved in the evasion of the host immune response. E. granulosus exists as a number of strains (G1–G10) that differ in biological characteristics. ... -
Identification of newly synthesized proteins by Echinococcus granulosus protoscoleces upon induction of strobilation
Debarba, João Antonio; Monteiro, Karina Mariante; Moura, Hércules; Barr, John Robert; Ferreira, Henrique Bunselmeyer; Zaha, Arnaldo (2015) [Artigo de periódico] -
In house colorimetric reverse hybridisation assay for detection of the mutation most frequently associated with resistance to isoniazid in Mycobacterium tuberculosis
Verza, Mirela; Maschmann, Raquel de Abreu; Silva, Marcia Susana Nunes; Dalla Costa, Elis Regina; Ribeiro, Marta Osório; Rosso, Franciele; Suffys, Philip Noel; Tortoli, Enrico; Marcelli, Fiorella; Zaha, Arnaldo; Rossetti, Maria Lucia Rosa (2009) [Artigo de periódico] -
In search of essentiality: mollicute-specific genes shared by twelve genomes
Souza, Rangel Celso; Almeida, Darcy Fontoura de; Zaha, Arnaldo; Morais, David Anderson de Lima; Vasconcelos, Ana Tereza Ribeiro de (2007) [Artigo de periódico] -
Insights on the virulence of swine respiratory tract mycoplasmas through genome-scale metabolic modeling
Ferrarini, Mariana Galvão; Siqueira, Franciele Maboni; Mucha, Scheila Gabriele; Palama, Tony L.; Jobard, Élodie; Herrmann, Bénédicte Elena; Vasconcelos, Ana Tereza Ribeiro de; Tardy, Florence; Schrank, Irene Silveira; Zaha, Arnaldo; Sagot, Marie France (2016) [Artigo de periódico]Background: The respiratory tract of swine is colonized by several bacteria among which are three Mycoplasma species: Mycoplasma flocculare, Mycoplasma hyopneumoniae and Mycoplasma hyorhinis. While colonization by M. ... -
Isolation and characterization of microsatellites from the tapeworm Echinococcus granulosus
Bartholomei-Santos, Marlise Ladvocat; Heinzelmann, Larissa Schemes; Oliveira, R. P.; Chemale, Gustavo; Gutierrez, Ariana M.; Kamenetzky, Laura; Haag, Karen Luisa; Zaha, Arnaldo (2003) [Artigo de periódico]The Echinococcus granulosus genome was searched for microsatellites using 8 different repeated oligonucleotides as probes (GT15, CT15, AT15, CG15, CAT10, CAA10, CGG10 and CATA10). Southern blot experiments revealed that ...