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dc.contributor.authorGonçalves, Fabiany da Costapt_BR
dc.contributor.authorLuk, Frankapt_BR
dc.contributor.authorKorevaar, Sander S.pt_BR
dc.contributor.authorBouzid, Rachidpt_BR
dc.contributor.authorPaz, Ana Helena da Rosapt_BR
dc.contributor.authorIglesias, Carmen Lópezpt_BR
dc.contributor.authorBaan, Carla C.pt_BR
dc.contributor.authorMerino, Anapt_BR
dc.contributor.authorHoogduijn, Martin J.pt_BR
dc.date.accessioned2018-04-26T02:33:33Zpt_BR
dc.date.issued2017pt_BR
dc.identifier.issn2045-2322pt_BR
dc.identifier.urihttp://hdl.handle.net/10183/175085pt_BR
dc.description.abstractMesenchymal stromal cells (MSC) are a promising therapy for immunological disorders. However, culture expanded MSC are large and get trapped in the capillary networks of the lungs after intravenous infusion, where they have a short survival time. Hypothetically, living cells are a risk for tumor formation. To reduce risks associated with MSC infusion and improve the distribution in the body, we generated membrane particles (MP) of MSC and MSC stimulated with IFN-γ (MPγ). Tracking analysis and electron microscopy indicated that the average size of MP was 120 nm, and they showed a round shape. MP exhibited ATPase, nucleotidase and esterase activity, indicating they are enzymatically active. MP and MPγ did not physically interact with T cells and had no effect on CD4+ and CD8+ T cells proliferation. However, MP and MPγ selectively bound to monocytes and decreased the frequency of pro-inflammatory CD14+CD16+ monocytes by induction of selective apoptosis. MP and MPγ increased the percentage of CD90 positive monocytes, and MPγ but not MP increased the percentage of antiinflammatory PD-L1 monocytes. MPγ increased mRNA expression of PD-L1 in monocytes. These data demonstrate that MP have immunomodulatory properties and have potential as a novel cell-free therapy for treatment of immunological disorders.en
dc.format.mimetypeapplication/pdfpt_BR
dc.language.isoengpt_BR
dc.relation.ispartofScientific reports. London. Vol. 7 (Sep. 2017), 12100, [13 p.]pt_BR
dc.rightsOpen Accessen
dc.subjectCélulas mesenquimais estromaispt_BR
dc.titleMembrane particles generated from mesenchymal stromal cells modulate immune responses by selective targeting of pro-inflammatory monocytespt_BR
dc.typeArtigo de periódicopt_BR
dc.identifier.nrb001065696pt_BR
dc.type.originEstrangeiropt_BR


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