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dc.contributor.authorPaz, Ana Helena da Rosapt_BR
dc.contributor.authorRief, Stephaniept_BR
dc.contributor.authorSantos, Diogenes Santiagopt_BR
dc.contributor.authorWolf, Eckhardpt_BR
dc.contributor.authorPrelle, Katjapt_BR
dc.contributor.authorCirne Lima, Elizabeth Obinopt_BR
dc.date.accessioned2010-04-16T09:11:38Zpt_BR
dc.date.issued2003pt_BR
dc.identifier.issn1678-0345pt_BR
dc.identifier.urihttp://hdl.handle.net/10183/19796pt_BR
dc.description.abstractDuring the last few years, embryonic stem (ES) cells have been a new tool in cell biology which is very promising for the scientific community to develop new cell therapies. ES cells are the only cell type that can differentiate into derivates of the three primary germ layers, not only in vivo but also, and most important, in vitro. This so-called pluripotency has resulted in the field of stem cell technology going into overdrive, and the establishment of many protocols for optimal maintenance, culture, genetic transfection and in vitro differentiation. The first pluripotent cells had been derived from teratocarcinomas, maligne tumors, and showed some disadvantages. Therefore later embryonic stem cells, and now adult stem cells are getting special attention from the scientists. In this study, we established for the first time in our country, the prolonged culture of undifferentiated ES cells in vitro and the pointed induction of cell differentiation into specific cell types. It is the result of an international collaboration program supported by Brazil and Germany, CAPES and DAAD (PROBRAL). The well-established routine should be clearly demonstrated by the continuous culture and propagation of several mouse ES lines in vitro under specific culture conditions preventing differentiation. On the other hand, these ES cells were exposed to defined differentiation induction systems to obtain specialized cells as cardiogenic, neurogenic and myogenic cell types. This demonstrates the successful procedure to induce ES cell line differentiation. In this study, we established both routine systems, with and without differentiation. This results gave us competence and possibility to develop a series of different scientific approaches .en
dc.format.mimetypeapplication/pdfpt_BR
dc.language.isoengpt_BR
dc.relation.ispartofActa scientiae veterinariae. Porto Alegre, RS. Vol. 31, n. 1 (2003), p. 51-54pt_BR
dc.rightsOpen Accessen
dc.subjectEmbryonic stem cellen
dc.subjectMicrobiologia molecularpt_BR
dc.subjectCelulas : Manipulacao in vitropt_BR
dc.subjectCardiac cellen
dc.subjectNeuronal cellen
dc.subjectMyogenic cellen
dc.subjectR1 and D3 cell linesen
dc.titleMouse embryonic stem cells: the establishment of the system to produce differentiated cell types in vitropt_BR
dc.typeArtigo de periódicopt_BR
dc.identifier.nrb000415828pt_BR
dc.type.originNacionalpt_BR


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