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dc.contributor.authorPilar, Emily Ferreira Sallespt_BR
dc.contributor.authorZettler, Claudio Galleanopt_BR
dc.date.accessioned2020-07-23T03:40:42Zpt_BR
dc.date.issued2020pt_BR
dc.identifier.issn2055-091Xpt_BR
dc.identifier.urihttp://hdl.handle.net/10183/212358pt_BR
dc.description.abstractBackground: Immunohistochemistry (IHC) is a method of identifying proteins in cells or tissues that is useful for diagnosis and research. In modern pathology, it has assumed an important supporting role in the molecular diagnosis of certain neoplasia, with an exponential contribution in personalized medicine. Automation in immunohistochemistry contributes to reduced test variability through standardization. The transition to automation is a process, and for a laboratory that already has a collection of stockconcentrated primary antibodies, ideally, these antibodies will be compatible with the chosen automated method, as antibodies are of high value to be discarded as a consequence of migration to automated immunohistochemistry. Methods: 78 concentrated antibodies were tested for use in Ventana Medical Systems’ Benchmark XT automation platform. Thirty-one human tissue samples containing the antigens of interest were used as positive control. Results: All antibodies tested showed good performance, indicating the feasibility of using these antibodies concentrated in the automation platform in question. The protocol most frequently used was the one with antigenic retrieval with Cell Conditioning 1 for 60 minutes and incubation in the primary antibody for 32 minutes at 42ºC. The dilutions of the primary antibodies in automation ranged from 1:20 to 1:4000. Conclusion: Under the aforementioned conditions, it was possible to take advantage of the portfolio of concentrated antibodies present in the laboratory at the time of transition from manual to automated immunohistochemistry.en
dc.format.mimetypeapplication/pdfpt_BR
dc.language.isoengpt_BR
dc.relation.ispartofJournal of histology and histopathology. Telangana, India. Vol. 7 (2020), Article 4, 11 p.pt_BR
dc.rightsOpen Accessen
dc.subjectAutomationen
dc.subjectImuno-histoquímicapt_BR
dc.subjectAnticorpospt_BR
dc.subjectImmunohistochemistryen
dc.subjectStandardizationen
dc.subjectTécnicas de laboratório clínicopt_BR
dc.subjectAutomação laboratorialpt_BR
dc.subjectConcentrated antibodiesen
dc.titleStandardization of concentrated antibodies for use in automated immunohistochemistrypt_BR
dc.typeArtigo de periódicopt_BR
dc.identifier.nrb001114964pt_BR
dc.type.originEstrangeiropt_BR


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