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dc.contributor.authorPaschoal, Aline Fernanda Lopespt_BR
dc.contributor.authorLuther, Anne-Mariept_BR
dc.contributor.authorJäkel, Helenpt_BR
dc.contributor.authorScheinpflug, Kathipt_BR
dc.contributor.authorMühldorfer, Kristinpt_BR
dc.contributor.authorBortolozzo, Fernando Pandolfopt_BR
dc.contributor.authorWaberski, Dagmarpt_BR
dc.date.accessioned2020-09-30T04:05:11Zpt_BR
dc.date.issued2020pt_BR
dc.identifier.issn1932-6203pt_BR
dc.identifier.urihttp://hdl.handle.net/10183/213886pt_BR
dc.description.abstractHypothermic storage of boar semen provides the possibility to omit antibiotics from semen extenders so long as sperm quality is maintained and bacterial growth prevented. The objective of this study was to determine an optimal cooling-rate frame for boar semen preserved at 5˚C in an antibiotic-free extender. Semen from eight boars extended in AndroStar® Premium was cooled from 30˚C to 5˚C using seven different cooling rates, ranging initially from 0.01 to 0.36˚C min–1 and reaching 5˚C between 2 h and 24 h after dilution. Sperm motility, membrane integrity, membrane fluidity, mitochondrial membrane potential and the response to the capacitation stimulus bicarbonate remained at a high level for 144 h at 5˚C when the semen was initially cooled in a cooling-rate frame ranging from 0.01 to 0.09˚C min-1 in the temperature zone from 30 to 25˚C, followed by 0.02 to 0.06˚C min–1 to 10˚C and 0.01 to 0.02˚C min-1 to the final storage temperature. A cooling rate of 0.07˚C min–1 in the temperature zone from 30 to 10˚C led to a reduced response to bicarbonate (P < 0.01) and fast cooling to 5˚C within 1 h with a cooling rate of 0.31˚C min–1 resulted in lower values (P > 0.05) of all sperm parameters. In a further experiment, slow cooling with a holding time of 6 h at 22˚C induced after 6 h storage a temporary increase in Escherichia coli of 0.5 × 103 to 2.4 × 103 CFU mL–1 in the sperm-free inoculated extender. Overall, the load of mesophilic bacteria in the stored semen was below 6 × 103 CFU mL–1, a level that is not regarded as critical for sperm quality. In conclusion, appropriate cooling protocols were established for the antibiotic-free storage of boar semen at 5˚C, allowing the application of hypothermic preservation in research and in artificial insemination.en
dc.format.mimetypeapplication/pdfpt_BR
dc.language.isoengpt_BR
dc.relation.ispartofPLOS ONE Journal. San Francisco, CA. Vol. 15, no. 6 (June 2020), e0234339, 14 p.pt_BR
dc.rightsOpen Accessen
dc.subjectPreservação do sêmenpt_BR
dc.subjectTemperatura baixapt_BR
dc.subjectQualidade do sêmenpt_BR
dc.subjectCrescimento bacterianopt_BR
dc.subjectInseminacao artificialpt_BR
dc.subjectSuínospt_BR
dc.titleDetermination of a cooling-rate frame for antibiotic-free preservation of boar semen at 5˚Cpt_BR
dc.typeArtigo de periódicopt_BR
dc.identifier.nrb001117010pt_BR
dc.type.originEstrangeiropt_BR


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