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Intercellular contact-dependent survival of human A549, NCI-H596 and NCI-H520 non-small cell lung carcinoma cell lines

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Intercellular contact-dependent survival of human A549, NCI-H596 and NCI-H520 non-small cell lung carcinoma cell lines

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Título Intercellular contact-dependent survival of human A549, NCI-H596 and NCI-H520 non-small cell lung carcinoma cell lines
Autor Castro, Mauro Antônio Alves
Schwartsmann, Gilberto
Moreira, Jose Claudio Fonseca
Abstract In the present study, we examined the relationship between cell phenotype and cell survival of three human non-small cell lung carcinoma cell lines (A549, NCI-H596 and NCI-H520). Cells in exponential growth at various densities were incubated for 24 h at 37ºC in a 5% CO2 humidified atmosphere and then exposed to UV radiation for 1 min (256 nm, 40 W, source-to-target distance 100 cm). After two days the surviving cells were quantified by sulforhodamine ß staining and DNA fragmentation assay. The differences in UV sensitivity at 60 x 103 cells/cm2 among the cell lines were not related to the proliferative state of the cells but to the extent of intercellular contact. In contrast to A549 and NCI-H596, irradiated NCI-H520 cells presented lower DNA fragmentation and an aggregated cell culture phenotype even prior to confluence, suggesting that a contact-effect mechanism provides further protection against UV radiation.
Contido em Brazilian journal of medical and biological research. Ribeirão Preto, SP. Vol. 34, no. 8 (Aug. 2001), p. 1007-1013
Assunto Carcinoma pulmonar de células não pequenas
Ciclo celular
Contagem de células
Fenótipo
Neoplasias pulmonares
Tolerância à radiação
[en] Cell density
[en] Cell phenotype
[en] Intercellular contact
[en] Non-small cell lung carcinoma
[en] UV radiation
Origem Nacional
Tipo Artigo de periódico
URI http://hdl.handle.net/10183/21138
Arquivos Descrição Formato
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