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Phenotyping and genotyping methods applied to investigate the relatedness of brazilian isolates of enterobacter cloacae

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Phenotyping and genotyping methods applied to investigate the relatedness of brazilian isolates of enterobacter cloacae

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Título Phenotyping and genotyping methods applied to investigate the relatedness of brazilian isolates of enterobacter cloacae
Autor Darini, A.L.C.
Magalhães, V.D.
Levy, C.L.
Barth, Afonso Luis
Coscina, A.L.
Abstract In order to evaluate the resolving power of several typing methods to identify relatedness among Brazilian strains of Enterobacter cloacae, we selected twenty isolates from different patients on three wards of a University Hospital (Orthopedics, Nephrology, and Hematology). Traditional phenotyping methods applied to isolates included biotyping, antibiotic sensitivity, phage-typing, and O-serotyping. Plasmid profile analysis, ribotyping, and macrorestriction analysis by pulsed-field gel electrophoresis (PFGE) were used as genotyping methods. Sero- and phage-typing were not useful since the majority of isolates could not be subtyped by these methods. Biotyping, antibiogram and plasmid profile permitted us to classify the samples into different groups depending on the method used, and consequently were not reliable. Ribotyping and PFGE were significantly correlated with the clinical epidemiological analysis. PFGE did not type strains containing nonspecific DNase. Ribotyping was the most discriminative method for typing Brazilian isolates of E. cloacae.
Contido em Brazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas. Ribeirão Preto, SP. Vol. 32, no. 9 (Sept. 1999), p. 1077-1081
Assunto Enterobacter cloacae
Enterobacterias
[en] Enterobacter cloacae
[en] Nosocomial infectio
[en] Phage-typing
[en] Pulsed-field gel electrophoresis
[en] Ribotyping
[en] Serotyping
Origem Nacional
Tipo Artigo de periódico
URI http://hdl.handle.net/10183/21158
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