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Lipid peroxidation and total radical-trapping potential of the lungs of rats submitted to chronic and sub-chronic stress

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Lipid peroxidation and total radical-trapping potential of the lungs of rats submitted to chronic and sub-chronic stress

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Título Lipid peroxidation and total radical-trapping potential of the lungs of rats submitted to chronic and sub-chronic stress
Autor Torres, Ronaldo Lopes
Gamaro, Giovana Duzzo
Torres, Iraci Lucena da Silva
Fontella, Fernanda Urruth
Silveira, Patrícia Pelufo
Moreira, Juliano Soares Rabello
Lacerda, Mariane Americo
Amoretti, José Ricardo Luz
Rech, Daniel
Dalmaz, Carla
Bello, Antonio Andrea
Abstract Exposure to stress induces a cluster of physiological and behavioral changes in an effort to maintain the homeostasis of the organism. Long-term exposure to stress, however, has detrimental effects on several cell functions such as the impairment of antioxidant defenses leading to oxidative damage. Oxidative stress is a central feature of many diseases. The lungs are particularly susceptible to lesions by free radicals and pulmonary antioxidant defenses are extensively distributed and include both enzymatic and non-enzymatic systems. The aim of the present study was to determine lipid peroxidation and total radical-trapping potential (TRAP) changes in lungs of rats submitted to different models of chronic stress. Adult male Wistar rats weighing 180-230 g were submitted to different stressors (variable stress, N = 7) or repeated restraint stress for 15 (N = 10) or 40 days (N = 6) and compared to control groups (N = 10 each). Lipid peroxidation levels were assessed by thiobarbituric acid reactive substances (TBARS), and TRAP was measured by the decrease in luminescence using the 2- 2'-azo-bis(2-amidinopropane)-luminol system. Chronic variable stress induced a 51% increase in oxidative stress in lungs (control group: 0.037 ± 0.002; variable stress: 0.056 ± 0.007, P < 0.01). No difference in TBARS was observed after chronic restraint stress, but a significant 57% increase in TRAP was presented by the group repeatedly restrained for 15 days (control group: 2.48 ± 0.42; stressed: 3.65 ± 0.16, P < 0.05). We conclude that different stressors induce different effects on the oxidative status of the organism.
Contido em Brazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas. Ribeirão Preto, SP. Vol. 37, no. 2 (Feb. 2004), p. 185-192
Assunto Bioquímica
[en] Free radicals
[en] Lungs
[en] Oxidative stress
[en] Stress
[en] TBARS
[en] TRAP
Origem Nacional
Tipo Artigo de periódico
URI http://hdl.handle.net/10183/21180
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